2nd tube of each strip: label with strip tube number (NOT sample number!).1st tube of each strip: label with 3-digit order label (assigned upon confirmation).Label your strip tubes as demonstrated by the scary dinosaurs Samples sent in 0.5 mL tubes, 1.5 mL tubes, or any other type of tubes are not compatible withĮach order will be assigned a 3-digit order label after the order has been confirmed through Samples should ONLY be sent in PCR strip tubes (or single PCR tubes if shipping single We mayĪttempt to re-sequence failed samples if your sample quality and quantity permits, but no refunds Requirements above prior to shipping to minimize chances of sequencing failure. We keep our prices low and turnaround times fast by trusting our customers to provide high-quality DNA. Our protocols have been shown to be tolerant to EDTA, but we recommend avoiding it if possible just in case. Genomic DNA, excess RNA, degraded plasmids) will not yield satisfactory data and may result On the target plasmid being the dominant species. Unlike Sanger sequencing, which uses a primer to target specific sequences, we sequence everything in the sample, so we rely Nanodrop values may adversely affect the library prep and/or sequencing reactions, possibly Sending samples at too high OR too low concentration based on Widely depending on the specific composition of your sample, so there is no standard way to The extent to which Nanodrop is off varies Quantification due to lower accuracy of the method. Nanodrop and other spectrophotometric instruments are NOT ADEQUATE for DNA The volume listed is the minimum required, but it never hurts to send more in case some is lost to evaporation. Please normalize as closely as possible to the listed concentration - higher concentrations are not better! Normalize your samples according to the table below. Use Qubit or another fluorometric method to measure your DNA concentrations, then

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